Project

Part:BBa_M31173:Design

Designed by: David Ying   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-03-06)

M13.1 from HpaI to BamHI, for reals this time!


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

My main objective was to move the promoter for g8 closer to its ORF, but in the process I had to unstuff various genetic elements involving g7 and g9.

I did not unstuff a few elements, like g7 RBS from g5 ORF because I did not think it was crucial to my design. I did not unstuff g3 promoter from g8 ORF because I thought this could introduce a confounding variable. I was also reluctant to change the DNA sequence of g8 ORF at all, since g8 was crucial to my study.

I also inserted single restriction sites at places where I had well defined parts, usually to section entire genes including regulatory elements from each other.

Source

M13K07 bacteriophage genome

References